| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Thermotoga maritima | AAAGTGGCGAACGAGGCT | TCGCGAGCCAGACAGATT | 59.57 | 58.71 | 268 | 112 |
100.00%
|
100.00%
|
| Primer 42 | Thermotoga maritima | TGGCCACTGCAACAGACA | ACCTGCTGACCAAAAGCGT | 59.41 | 60.15 | 264 | 112 |
100.00%
|
100.00%
|
| Primer 43 | Thermotoga maritima | TCTTCAAAGCGCTGGCGT | TCATTCCCTCCCTTCGGCT | 60.28 | 60.31 | 254 | 112 |
100.00%
|
100.00%
|
| Primer 44 | Thermotoga maritima | ACGCACAGTGAAACGGGT | CGATTGCAACTGCGTTCGT | 59.81 | 59.80 | 271 | 112 |
100.00%
|
100.00%
|
| Primer 45 | Thermotoga maritima | ACACCGACAAGTGCGCTT | TCTCGAGTGTCTCCTGCCT | 60.20 | 59.62 | 190 | 111 |
100.00%
|
100.00%
|
| Primer 46 | Thermotoga maritima | ACGACGCTCAAACGACTGA | TTTGCGACAACCTCCGGA | 59.64 | 59.18 | 172 | 110 |
100.00%
|
100.00%
|
| Primer 47 | Thermotoga maritima | TGTCACCGCTCTCATCACG | ACGGATCGTTCACGCTGA | 59.79 | 59.04 | 260 | 110 |
100.00%
|
100.00%
|
| Primer 48 | Thermotoga maritima | ACGAGCCAGAGGGTGTGAA | TGTAGCGTTTGCCGTCGA | 60.84 | 59.66 | 183 | 109 |
100.00%
|
100.00%
|
| Primer 49 | Thermotoga maritima | GATGAGCGGTGTGGAAGACA | TGTAGCGTTTGCCGTCGA | 60.04 | 59.66 | 152 | 109 |
100.00%
|
100.00%
|
| Primer 50 | Thermotoga maritima | TGATGAGCGGTGTGGAAGAC | TGTAGCGTTTGCCGTCGA | 60.04 | 59.66 | 153 | 109 |
100.00%
|
100.00%
|